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Lithium acetate borate buffer

Purpose

Low cost buffer for fast agarose gel electrophoresis and making agarose gels

Materials and Recipe

Ingredient 1X working concentration 25X stock concentration Catalog No
Lithium acetate dihydrate 10mM 250mM Sigma, L4158-100G
Boric acid 10mM 250mM RPI, B32025-500

Procedure

For 1L of 25X stock
Lithium acetate dihydrate 25.5g
Boric acid 15.5g
  • Bring up 1L with dH2O.
  • Dilute to 1x and use it to make the gel and to run the gel. Do not mix buffer systems.
  • Ethidium bromide can be added to the gel.
  • Run the gel at 15-20 V/cm (possibly higher) for 15-20 minutes (it’s good to set a timer).

For 1L of 1X working solution

Component Weight
Lithium acetate dihydrate 1g
Boric acid 0.6g
  • Bring up 1L with dH2O.

Reference