10X SDS PAGE buffer¶
Use for running Bio-Rad Mini-PROTEAN TGX gels and equivalents.
| Component | Amount | Final (10X) Concentration |
|---|---|---|
| Tris Base | 30.3 g | 250 mM |
| Glycine | 144.1 g | 1.92 M |
| SDS | 10 g | 1 % |
| Water | to 1 L |
- Dissolve ingredients in 1 L of water.
- To make 1X SDS PAGE buffer, dilute 10-fold with water.