Tbe buffer
Purpose¶
Buffer for both agarose and PAGE RNA gel electrophoresis.
Materials and Recipe¶
| Ingredient | 0.5X working concentration | 1L of 5X stock | Catalog No |
|---|---|---|---|
| Tris base | 45mM | 54g | Sigma, T1503-10KG |
| Boric acid | 45mM | 27.5g | RPI, B32025-500 |
| 0.5M EDTA, pH 8.0 | 1mM | 20ml | Fisher, PRV4231 |
Procedure¶
For 1L of 5X stock¶
- Add the Tris base and boric acid to 500 ml of DEPC H2O.
- Add 20ml of the the 0.5M EDTA.
- Bring up 1L with DEPC H2O.
- Shake to dissolve.
- The pH of the concentrated stock should be \~8.3.