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Tbst

Purpose

Buffer for antibody dilutions and Western blot washes.

Materials (20X TBS)

Component 1X (final) concentration 20X concentration Amount for 1L stock
NaCl 137 mM 2.74 M 160 g
KCl 2.7 mM 54 mM 4 g
Tris base 19 mM 380 mM 46 g

TBST contains 19 mM Tris, 137 mM NaCl, 2.7 mM KCl, and 0.1% Tween-20.

Procedure

  1. Dissolve the above components in 1 L of water.
  2. To prepare 1X TBS, dilute 20-fold with water.
  3. TO prepare 1X TBST, dilute 20-fold with water, and add 1 mL of Tween-20 for every liter of 1X TBS.