1X Novablot Transfer Buffer¶
Use this for both semi-dry (preferred) or wet transfers.
- 5.82 g Tris Base (MW=121.1)
- 2.93 g Glycine
- 200 mL Methanol
- 0.375 g SDS
- ddH2O up to 1 L
Add methanol and SDS last.
10X Towbin Buffer¶
Alternate transfer buffer.
| Component | Amount | Final (10X) Concentration |
|---|---|---|
| Tris Base | 30.3 g | 250 mM |
| Glycine | 144.1 g | 1.92 M |
| SDS | 1 g | 0.1 % |
| Water | to 1 L |
- Mix dry reagents with water and adjust volume to 1 L.
- To make 1X transfer buffer, combine 100 mL 10X Towbin Buffer, 100 mL MeOH, and 800 mL water. This transfer buffer is 25 mM Tris, 192 mM glycine, 0.01% SDS, 10% MeOH.