We prefer to store DNA constructs as plasmids instead of E. coli glycerol stocks.
Never let plasmid stocks drop below 20µl since we will not be able to re-create them without lot of effort.
Print plasmid number and date on a round sticker from the lab minicircle printer, and attach it to the 1.5ml tube.
Stock plasmid minipreps in your -20C freezer.
If your plasmid runs below 20µl, then transform it again, plate the full volume on a plate, and miniprep after 10-12 hours, and add it back to the tube.
Store glycerol stocks in your designated -80C box in a cryo vial with just the number printed in large font on the top (eg. print 80 instead of ecAS80). The large font is necessary to be able to read the vial easily when we pull tubes out.
Typically, we keeyp E. coli glycerol stocks corresponding to the following plasmids:
Plasmids that are used in publications.
Plasmids that we get from Addgene or other labs.
Plasmids that you use repeatedly (for eg. for lentivirus packaging, Bxb1 integration, CRISPR).
Store DMSO stocks in your designated LN2 box in a cryo vial with just the number printed in large font on the top (eg. print 80 instead of hsAS80). The large font is necessary to be able to read the vial easily when we pull tubes out.
When you leave the lab, we will aim to store only the following types of cell lines and discard the rest:
Cell lines used in publications.
Cell lines gotten from ATCC or other labs.
Cell lines that are hard to create (for example, any clonal line with gene deletion or tagging).
Cell lines used repeatedly (for eg. landing pad lines).