Validated antibodies for Western blots

Antibodies we have bought that work for chemiluminescent Western blots

Vendor	Catalog No.	Reactivity	Antigen	Host	Dilution	Location	Notes
Sigma	T8203	Human	α-Tubulin	Mouse	1:10,000	-20 °C	Good loading control
ProteinTech	60035-1-Ig	Human	ATF4	Mouse	1:5,000	-20 °C	Seen after treatment w/ thapsigargin
CST	5324	Human	eIF2α	Rabbit	1:5,000	-20 °C	Recognizes both P- and non P-
LifeTech	A301843AT	Human	GCN1	Rabbit	1:5,000	4 °C	(a.k.a. GCN1L1)
			Ribosomal Proteins:
CST	14568S	Human	RPL5	Rabbit	1:1,000	-20 °C
ProteinTech	68110-1-Ig	Human	RPL12	Mouse	1:10,000	-20 °C
ProteinTech	68056-1-Ig	Human	RPLP0	Mouse	1:20,000	-20 °C
SantaCruz	sc-100836	Human	RPS19	Rabbit	1:1,000	4 °C	Crummy signal
Bethyl Labs	A303-794A	Human	RPS2	Rabbit	1:5,000	4 °C

Antibodies we have bought that don't seem to work well (or at all)

Vendor	Catalog No.	Reactivity	Antigen	Host	Dilution	Location	Notes
		Human	Phospho-eIF2α	Rabbit	>1:1,000	-20 °C	Have not yet seen any signal

Antibodies we have bought that are not yet validated

Vendor	Catalog No.	Reactivity	Antigen	Host	Dilution	Location	Notes
			Puromycin	Mouse		4 °C