Validated antibodies for Western blots

Antibodies against human proteins that we have bought that work for chemiluminescent Western blots

Vendor	Catalog No.	Reactivity	Antigen	Host	Size	Dilution	Location	Notes
LifeTech	A301843AT	Human	GCN1	Rabbit	200 kD	1:5,000	+4 °C	(a.k.a. GCN1L1)
Sigma	T8203	Human	α-Tubulin	Mouse	50 kD	1:5,000	-20 °C	Good loading control
CST	11815	Human	ATF4	Rabbit	50 kD	1:1,000	-20 °C	Use Femto reagent
CST	5324	Human	eIF2α	Rabbit	38 kD	1:5,000	-20 °C	Recognizes both P- and non P-
CST	3398	Human	Phospho-eIF2	Rabbit	38 kD	1:2,000	-20 °C	Use Femto reagent
			Ribosomal Proteins:
ProteinTech	68056-1-Ig	Human	RPLP0	Mouse	34 kD	1:20,000	-20 °C
CST	14568S	Human	RPL5	Rabbit	34 kD	1:1,000	-20 °C
Bethyl Labs	A303-794A	Human	RPS2	Rabbit	30 kD	1:5,000	+4 °C
ProteinTech	68110-1-Ig	Human	RPL12	Mouse	21 kD	1:10,000	-20 °C
SantaCruz	sc-100836	Human	RPS19	Rabbit	15 kD	1:1,000	+4 °C	Crummy signal, but it works

Antibodies we have bought that don't seem to work well (or at all)

Vendor	Catalog No.	Reactivity	Antigen	Host	Size	Dilution	Location	Notes
ProteinTech	60035-1-Ig	Human	ATF4	Mouse	50 kD	1:5,000	-20 °C	No specific bands observed after treatment w/ thapsigargin

Antibodies we have bought that are not yet validated

Vendor	Catalog No.	Reactivity	Antigen	Host	Dilution	Location	Notes
Sigma	MABE343	n/a	Puromycin	Mouse	Unknown	+4 °C	Not tried yet