Validated antibodies for Western blots

Antibodies against human proteins that we have bought that work for chemiluminescent Western blots

Vendor	Catalog No.	Reactivity	Antigen	Host	Size	Dilution	Location	Notes
			Proteins:
LifeTech	A301843AT	Human	GCN1	Rabbit	200 kD	1:5,000	+4 °C	(a.k.a. GCN1L1)
Sigma	T8203	Human	α-Tubulin	Mouse	50 kD	1:2,000	-20 °C	Good loading control, but weak signal
CST	11815	Human	ATF4	Rabbit	50 kD	1:1,000	-20 °C	Use mixed Femto:Pico reagent
CST	5324	Human	eIF2α	Rabbit	38 kD	1:10,000	-20 °C
CST	8690	Human	p38 MAP Kinase	Rabbit	38 kD	1:5,000	-20 °C
			Phosphoproteins:
CST	3398	Human	Phospho-eIF2 (S51)	Rabbit	38 kD	1:2,000	-20 °C	Use mixed Femto:Pico reagent, and BSA for blocking
BioLegend	690201	Human	Phospho-p38 (T180/Y182)	Mouse	38 kD	1:2,000	4 °C	Use mixed Femto:Pico reagent, and BSA for blocking
			Ribosomal Proteins:
ProteinTech	68056-1-Ig	Human	RPLP0	Mouse	34 kD	1:20,000	-20 °C
CST	14568S	Human	RPL5	Rabbit	34 kD	1:5,000	-20 °C
Bethyl Labs	A303-794A	Human	RPS2	Rabbit	30 kD	1:5,000	+4 °C
Bethyl Labs	A303-840A	Human	RPS3	Rabbit	30 kD	1:10,000	+4 °C
ProteinTech	68110-1-Ig	Human	RPL12	Mouse	21 kD	1:10,000	-20 °C
SantaCruz	sc-100836	Human	RPS19	Rabbit	15 kD	1:1,000	+4 °C	Crummy signal, but it works
			Other:
Proteintech	HRP-66002	n/a	GFP	Mouse	n/a	1:20,000	-20 °C	HRP-conjugated for direct GFP detection
Sigma	MABE343	n/a	Puromycin	Mouse	n/a	1:10,000	+4 °C	Works for measuring nascent protein levels

Antibodies we have bought that don't seem to work

Vendor	Catalog No.	Reactivity	Antigen	Host	Size	Dilution	Location	Notes
ProteinTech	60035-1-Ig	Human	ATF4	Mouse	50 kD	1:5,000	-20 °C	No specific bands observed after treatment w/ thapsigargin

Antibodies we have bought that are not yet validated

Vendor	Catalog No.	Reactivity	Antigen	Host	Size	Dilution	Location	Notes
Bethyl Labs	A301-993A-T	Human	ZAK kinase	Rabbit			+4 °C