| Heungwon |
Homemade Phusion, Superscript, T7RNAP |
Monthly |
| Patrick |
Aliquots of Gibson master mix |
Every 2 or 3 months |
| Patrick |
Agarose buffers |
Every 6 months |
| Patrick |
Western blot buffers |
Every 6 months |
| Jamie |
All tissue culture jobs |
Weekly |
| Maria |
Aliquots of Antibiotics |
Monthly |
| Rachael |
Order lab supplies and lab label |
Weekly |
| Rachael |
Check common 4C, -20C, -80C, -140C for defrosting and organization |
Every 2 weeks |
| Matthew |
Autoclaved glass beads, cryovials + caps (separate) for glycerol stocks |
Minimum 2 bottles, monthly |
| Matthew |
50% glucose (100ml), 50% glycerol (100ml) |
Minimum 2 bottles, check weekly |
| Heather |
Agar plates: Carb50, Chl25, Kan25 |
Minimum 1 sleeve, check weekly |
| Heather |
LB buffer (500ml) and SOB (100mL) |
Minimum 2 bottles, check weekly |
| Heather |
Make competent cells (Chemical and Electrocompetent) |
As necessary, check weekly |