| Heungwon |
Homemade Phusion, Superscript, T7RNAP |
Monthly |
| Heungwon |
Check and make 1kb ladder |
Minimum for 20 gel run, check weekly |
| Heungwon |
Replenish PCR supplies |
Minimum 2 bags of caps / tubes, check weekly |
| Heungwon |
50% glucose (100ml), 50% glycerol (100ml) |
Minimum 2 bottles, check weekly |
| Katharine |
Make competent cells (Chemical and Electrocompetent) |
As necessary, check weekly |
| Katharine |
YEP (1000/500ml) |
Minimum 2 bottles, check weekly |
| Katharine |
Agar plates: YEPD, SC-Ura, SC-Leu |
Minimum 1 sleeve, check weekly |
| Katharine |
Clean chemical hood and label waste |
Weekly |
| Katharine |
Clean chemical weighing area |
Monthly |
| Patrick |
Aliquots of Gibson master mix |
Every 2 or 3 months |
| Patrick |
Agarose buffers |
Every 6 months |
| Patrick |
Western blot buffers |
Every 6 months |
| Patrick |
Clean gel area and Nanodrop area |
Every 2 months |
| Jamie |
All tissue culture jobs |
Weekly |
| Jamie |
Fill 37C and 42C water baths |
Weekly |
| Maria |
fill 190 proof ethanol |
Every 6 months |
| Maria |
Aliquots of Antibiotics |
Monthly |
| Rachael |
Check common lab drawers for supplies replenishing and lab label |
Weekly |
| Rachael |
Check common 4C, -20C, -80C, -140C for defrosting and organization |
Every 2 weeks |
| Rachael |
Put out glass pipettes for washing |
Weekly |
| Matthew |
Autoclaved glass beads, cryovials + caps (separate) for glycerol stocks |
Minimum 2 bottles, monthly |
| Matthew |
Put away washed glassware |
Every ~2 days |
| Shreya |
Agar plates: Carb50, Chl25, Kan25 |
Minimum 1 sleeve, check weekly |
| Shreya |
LB buffer (500ml) and SOB (100mL) |
Minimum 2 bottles, check weekly |